Activation Assay:Article Title: Optimal CXCR5 expression during Tfh maturation involves the Bhlhe40-Pou2af1 axis.
Article Snippet: Compound fisher healthcare Cat#23-730-571 VWR® Superfrost® Plus Micro Slide, Premium Avantor Cat#48311-703 PBS, pH 7.4 ThermoFisher Cat#10010031 Fluoromount G Southern Biotech Cat#0100-01 Coating buffer (pH = 9.5) BD Cat#51-2713KC Stop Solution invitrogen Cat#SS04 normal mouse serum Jackson immuno Research Cat#015-000-120 (Continued on next page) Cell Reports 44, 116470, November 25, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER normal rat serum Jackson immuno Research Cat012-000-120 IL-2 R&D systems Cat#401-ML IL-12 PeproTech Cat#210-12 IL-4 R&D Systems Cat#404-ML TGFβ1 R&D systems Cat#7666-MB IL-6 PeproTech Cat#216-16 IL-1β R&D systems Cat#401-ML Fixable Viability Dye eFluor TM 506 eBioscience Cat# 65-0866-18 Fixable Viability Dye eFluor TM 780 eBioscience Cat#65-0865-14 Dynabeads TM Mouse T-Activator CD3/ CD28 for T cell Expansion and Activation Thermofisher Cat#11452D UltraComp eBeads TM Compensation Bead Thermofisher Cat#01-2222-41 Phorbol 12-myristate 13-acetate Sigma Cat#P8139-5MG Ionomycin calcium salt from Streptomyces conglobatus Sigma Cat#I0634-5MG Monensin Solution (1000×) eBioscience Cat#00-4505-51 Critical commercial assays IgG1 Mouse Uncoated ELISA Kit with Plates invitrogen Cat#88-50410-86 IgG2c Mouse Uncoated ELISA Kit with Plates invitrogen Cat#88-50670-22 IgG (Total) Mouse Uncoated ELISA Kit with Plates invitrogen Cat#88-50400-86 miRNeasy micro kit Qiagen Cat#217084 DNase set Qiagen Cat#79254 QubitTM RNA High Sensitivity (HS), Assay Kit ThermoFisher Cat#Q10210 QubitTM dsDNA Broad Rang (BR) Assay Kit ThermoFisher Cat#Q32850 Dynabeads mRNA DIR ECT kit Ambion Life Technologies Cat#61012 EasySep TM Mouse Naive CD4 + T cell Isolation Kit STEMCELL Cat#19765 BD OptEIA TM Reagent Set Bt B BD Cat#550534 Foxp3 staining buffer set eBioscience Cat#00-5523-00 Deposited data RNA-Seq Raw and analyzed data This paper GSE247137 ChIP-Seq Raw and analyzed data This paper GSE247137 Experimental models: Organisms/strains Mouse: Pou2af1 − /− , This paper N/A Mouse: Pou2af1-HA-tdTomato This paper N/A Mouse: Bhlhe40 − /− This paper N/A Mouse: Bhlhe40-V5 This paper N/A Mouse: Bhlhe40 fl/fl –CD4Cre This paper Yu et al. 24 Mouse: Pou2af1 tm1a(KOMP)Wtsi the University of California, Davis, KOMP Repository www.mmrrc.org Mouse: CD45.1 + NIAID-Taconic repository Line 7 Mouse: Rag1 − /− NIAID-Taconic repository Line 146 Mouse: TCRα − /− NIAID-Taconic repository Line 98 Mouse: Cas9 JAXSON JAX 026179 Mouse: OT-II NIAID-Taconic repository Line 187 (Continued on next page) 18 Cell Reports 44, 116470, November 25, 2025 ..
Enzyme-linked Immunosorbent Assay:Article Title: Optimal CXCR5 expression during Tfh maturation involves the Bhlhe40-Pou2af1 axis.
Article Snippet: Compound fisher healthcare Cat#23-730-571 VWR® Superfrost® Plus Micro Slide, Premium Avantor Cat#48311-703 PBS, pH 7.4 ThermoFisher Cat#10010031 Fluoromount G Southern Biotech Cat#0100-01 Coating buffer (pH = 9.5) BD Cat#51-2713KC Stop Solution invitrogen Cat#SS04 normal mouse serum Jackson immuno Research Cat#015-000-120 (Continued on next page) Cell Reports 44, 116470, November 25, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER normal rat serum Jackson immuno Research Cat012-000-120 IL-2 R&D systems Cat#401-ML IL-12 PeproTech Cat#210-12 IL-4 R&D Systems Cat#404-ML TGFβ1 R&D systems Cat#7666-MB IL-6 PeproTech Cat#216-16 IL-1β R&D systems Cat#401-ML Fixable Viability Dye eFluor TM 506 eBioscience Cat# 65-0866-18 Fixable Viability Dye eFluor TM 780 eBioscience Cat#65-0865-14 Dynabeads TM Mouse T-Activator CD3/ CD28 for T cell Expansion and Activation Thermofisher Cat#11452D UltraComp eBeads TM Compensation Bead Thermofisher Cat#01-2222-41 Phorbol 12-myristate 13-acetate Sigma Cat#P8139-5MG Ionomycin calcium salt from Streptomyces conglobatus Sigma Cat#I0634-5MG Monensin Solution (1000×) eBioscience Cat#00-4505-51 Critical commercial assays IgG1 Mouse Uncoated ELISA Kit with Plates invitrogen Cat#88-50410-86 IgG2c Mouse Uncoated ELISA Kit with Plates invitrogen Cat#88-50670-22 IgG (Total) Mouse Uncoated ELISA Kit with Plates invitrogen Cat#88-50400-86 miRNeasy micro kit Qiagen Cat#217084 DNase set Qiagen Cat#79254 QubitTM RNA High Sensitivity (HS), Assay Kit ThermoFisher Cat#Q10210 QubitTM dsDNA Broad Rang (BR) Assay Kit ThermoFisher Cat#Q32850 Dynabeads mRNA DIR ECT kit Ambion Life Technologies Cat#61012 EasySep TM Mouse Naive CD4 + T cell Isolation Kit STEMCELL Cat#19765 BD OptEIA TM Reagent Set Bt B BD Cat#550534 Foxp3 staining buffer set eBioscience Cat#00-5523-00 Deposited data RNA-Seq Raw and analyzed data This paper GSE247137 ChIP-Seq Raw and analyzed data This paper GSE247137 Experimental models: Organisms/strains Mouse: Pou2af1 − /− , This paper N/A Mouse: Pou2af1-HA-tdTomato This paper N/A Mouse: Bhlhe40 − /− This paper N/A Mouse: Bhlhe40-V5 This paper N/A Mouse: Bhlhe40 fl/fl –CD4Cre This paper Yu et al. 24 Mouse: Pou2af1 tm1a(KOMP)Wtsi the University of California, Davis, KOMP Repository www.mmrrc.org Mouse: CD45.1 + NIAID-Taconic repository Line 7 Mouse: Rag1 − /− NIAID-Taconic repository Line 146 Mouse: TCRα − /− NIAID-Taconic repository Line 98 Mouse: Cas9 JAXSON JAX 026179 Mouse: OT-II NIAID-Taconic repository Line 187 (Continued on next page) 18 Cell Reports 44, 116470, November 25, 2025 ..
Cell Isolation:Article Title: Optimal CXCR5 expression during Tfh maturation involves the Bhlhe40-Pou2af1 axis.
Article Snippet: Compound fisher healthcare Cat#23-730-571 VWR® Superfrost® Plus Micro Slide, Premium Avantor Cat#48311-703 PBS, pH 7.4 ThermoFisher Cat#10010031 Fluoromount G Southern Biotech Cat#0100-01 Coating buffer (pH = 9.5) BD Cat#51-2713KC Stop Solution invitrogen Cat#SS04 normal mouse serum Jackson immuno Research Cat#015-000-120 (Continued on next page) Cell Reports 44, 116470, November 25, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER normal rat serum Jackson immuno Research Cat012-000-120 IL-2 R&D systems Cat#401-ML IL-12 PeproTech Cat#210-12 IL-4 R&D Systems Cat#404-ML TGFβ1 R&D systems Cat#7666-MB IL-6 PeproTech Cat#216-16 IL-1β R&D systems Cat#401-ML Fixable Viability Dye eFluor TM 506 eBioscience Cat# 65-0866-18 Fixable Viability Dye eFluor TM 780 eBioscience Cat#65-0865-14 Dynabeads TM Mouse T-Activator CD3/ CD28 for T cell Expansion and Activation Thermofisher Cat#11452D UltraComp eBeads TM Compensation Bead Thermofisher Cat#01-2222-41 Phorbol 12-myristate 13-acetate Sigma Cat#P8139-5MG Ionomycin calcium salt from Streptomyces conglobatus Sigma Cat#I0634-5MG Monensin Solution (1000×) eBioscience Cat#00-4505-51 Critical commercial assays IgG1 Mouse Uncoated ELISA Kit with Plates invitrogen Cat#88-50410-86 IgG2c Mouse Uncoated ELISA Kit with Plates invitrogen Cat#88-50670-22 IgG (Total) Mouse Uncoated ELISA Kit with Plates invitrogen Cat#88-50400-86 miRNeasy micro kit Qiagen Cat#217084 DNase set Qiagen Cat#79254 QubitTM RNA High Sensitivity (HS), Assay Kit ThermoFisher Cat#Q10210 QubitTM dsDNA Broad Rang (BR) Assay Kit ThermoFisher Cat#Q32850 Dynabeads mRNA DIR ECT kit Ambion Life Technologies Cat#61012 EasySep TM Mouse Naive CD4 + T cell Isolation Kit STEMCELL Cat#19765 BD OptEIA TM Reagent Set Bt B BD Cat#550534 Foxp3 staining buffer set eBioscience Cat#00-5523-00 Deposited data RNA-Seq Raw and analyzed data This paper GSE247137 ChIP-Seq Raw and analyzed data This paper GSE247137 Experimental models: Organisms/strains Mouse: Pou2af1 − /− , This paper N/A Mouse: Pou2af1-HA-tdTomato This paper N/A Mouse: Bhlhe40 − /− This paper N/A Mouse: Bhlhe40-V5 This paper N/A Mouse: Bhlhe40 fl/fl –CD4Cre This paper Yu et al. 24 Mouse: Pou2af1 tm1a(KOMP)Wtsi the University of California, Davis, KOMP Repository www.mmrrc.org Mouse: CD45.1 + NIAID-Taconic repository Line 7 Mouse: Rag1 − /− NIAID-Taconic repository Line 146 Mouse: TCRα − /− NIAID-Taconic repository Line 98 Mouse: Cas9 JAXSON JAX 026179 Mouse: OT-II NIAID-Taconic repository Line 187 (Continued on next page) 18 Cell Reports 44, 116470, November 25, 2025 ..
Staining:Article Title: Optimal CXCR5 expression during Tfh maturation involves the Bhlhe40-Pou2af1 axis.
Article Snippet: Compound fisher healthcare Cat#23-730-571 VWR® Superfrost® Plus Micro Slide, Premium Avantor Cat#48311-703 PBS, pH 7.4 ThermoFisher Cat#10010031 Fluoromount G Southern Biotech Cat#0100-01 Coating buffer (pH = 9.5) BD Cat#51-2713KC Stop Solution invitrogen Cat#SS04 normal mouse serum Jackson immuno Research Cat#015-000-120 (Continued on next page) Cell Reports 44, 116470, November 25, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER normal rat serum Jackson immuno Research Cat012-000-120 IL-2 R&D systems Cat#401-ML IL-12 PeproTech Cat#210-12 IL-4 R&D Systems Cat#404-ML TGFβ1 R&D systems Cat#7666-MB IL-6 PeproTech Cat#216-16 IL-1β R&D systems Cat#401-ML Fixable Viability Dye eFluor TM 506 eBioscience Cat# 65-0866-18 Fixable Viability Dye eFluor TM 780 eBioscience Cat#65-0865-14 Dynabeads TM Mouse T-Activator CD3/ CD28 for T cell Expansion and Activation Thermofisher Cat#11452D UltraComp eBeads TM Compensation Bead Thermofisher Cat#01-2222-41 Phorbol 12-myristate 13-acetate Sigma Cat#P8139-5MG Ionomycin calcium salt from Streptomyces conglobatus Sigma Cat#I0634-5MG Monensin Solution (1000×) eBioscience Cat#00-4505-51 Critical commercial assays IgG1 Mouse Uncoated ELISA Kit with Plates invitrogen Cat#88-50410-86 IgG2c Mouse Uncoated ELISA Kit with Plates invitrogen Cat#88-50670-22 IgG (Total) Mouse Uncoated ELISA Kit with Plates invitrogen Cat#88-50400-86 miRNeasy micro kit Qiagen Cat#217084 DNase set Qiagen Cat#79254 QubitTM RNA High Sensitivity (HS), Assay Kit ThermoFisher Cat#Q10210 QubitTM dsDNA Broad Rang (BR) Assay Kit ThermoFisher Cat#Q32850 Dynabeads mRNA DIR ECT kit Ambion Life Technologies Cat#61012 EasySep TM Mouse Naive CD4 + T cell Isolation Kit STEMCELL Cat#19765 BD OptEIA TM Reagent Set Bt B BD Cat#550534 Foxp3 staining buffer set eBioscience Cat#00-5523-00 Deposited data RNA-Seq Raw and analyzed data This paper GSE247137 ChIP-Seq Raw and analyzed data This paper GSE247137 Experimental models: Organisms/strains Mouse: Pou2af1 − /− , This paper N/A Mouse: Pou2af1-HA-tdTomato This paper N/A Mouse: Bhlhe40 − /− This paper N/A Mouse: Bhlhe40-V5 This paper N/A Mouse: Bhlhe40 fl/fl –CD4Cre This paper Yu et al. 24 Mouse: Pou2af1 tm1a(KOMP)Wtsi the University of California, Davis, KOMP Repository www.mmrrc.org Mouse: CD45.1 + NIAID-Taconic repository Line 7 Mouse: Rag1 − /− NIAID-Taconic repository Line 146 Mouse: TCRα − /− NIAID-Taconic repository Line 98 Mouse: Cas9 JAXSON JAX 026179 Mouse: OT-II NIAID-Taconic repository Line 187 (Continued on next page) 18 Cell Reports 44, 116470, November 25, 2025 ..
RNA sequencing:Article Title: Optimal CXCR5 expression during Tfh maturation involves the Bhlhe40-Pou2af1 axis.
Article Snippet: Compound fisher healthcare Cat#23-730-571 VWR® Superfrost® Plus Micro Slide, Premium Avantor Cat#48311-703 PBS, pH 7.4 ThermoFisher Cat#10010031 Fluoromount G Southern Biotech Cat#0100-01 Coating buffer (pH = 9.5) BD Cat#51-2713KC Stop Solution invitrogen Cat#SS04 normal mouse serum Jackson immuno Research Cat#015-000-120 (Continued on next page) Cell Reports 44, 116470, November 25, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER normal rat serum Jackson immuno Research Cat012-000-120 IL-2 R&D systems Cat#401-ML IL-12 PeproTech Cat#210-12 IL-4 R&D Systems Cat#404-ML TGFβ1 R&D systems Cat#7666-MB IL-6 PeproTech Cat#216-16 IL-1β R&D systems Cat#401-ML Fixable Viability Dye eFluor TM 506 eBioscience Cat# 65-0866-18 Fixable Viability Dye eFluor TM 780 eBioscience Cat#65-0865-14 Dynabeads TM Mouse T-Activator CD3/ CD28 for T cell Expansion and Activation Thermofisher Cat#11452D UltraComp eBeads TM Compensation Bead Thermofisher Cat#01-2222-41 Phorbol 12-myristate 13-acetate Sigma Cat#P8139-5MG Ionomycin calcium salt from Streptomyces conglobatus Sigma Cat#I0634-5MG Monensin Solution (1000×) eBioscience Cat#00-4505-51 Critical commercial assays IgG1 Mouse Uncoated ELISA Kit with Plates invitrogen Cat#88-50410-86 IgG2c Mouse Uncoated ELISA Kit with Plates invitrogen Cat#88-50670-22 IgG (Total) Mouse Uncoated ELISA Kit with Plates invitrogen Cat#88-50400-86 miRNeasy micro kit Qiagen Cat#217084 DNase set Qiagen Cat#79254 QubitTM RNA High Sensitivity (HS), Assay Kit ThermoFisher Cat#Q10210 QubitTM dsDNA Broad Rang (BR) Assay Kit ThermoFisher Cat#Q32850 Dynabeads mRNA DIR ECT kit Ambion Life Technologies Cat#61012 EasySep TM Mouse Naive CD4 + T cell Isolation Kit STEMCELL Cat#19765 BD OptEIA TM Reagent Set Bt B BD Cat#550534 Foxp3 staining buffer set eBioscience Cat#00-5523-00 Deposited data RNA-Seq Raw and analyzed data This paper GSE247137 ChIP-Seq Raw and analyzed data This paper GSE247137 Experimental models: Organisms/strains Mouse: Pou2af1 − /− , This paper N/A Mouse: Pou2af1-HA-tdTomato This paper N/A Mouse: Bhlhe40 − /− This paper N/A Mouse: Bhlhe40-V5 This paper N/A Mouse: Bhlhe40 fl/fl –CD4Cre This paper Yu et al. 24 Mouse: Pou2af1 tm1a(KOMP)Wtsi the University of California, Davis, KOMP Repository www.mmrrc.org Mouse: CD45.1 + NIAID-Taconic repository Line 7 Mouse: Rag1 − /− NIAID-Taconic repository Line 146 Mouse: TCRα − /− NIAID-Taconic repository Line 98 Mouse: Cas9 JAXSON JAX 026179 Mouse: OT-II NIAID-Taconic repository Line 187 (Continued on next page) 18 Cell Reports 44, 116470, November 25, 2025 ..
Chromatin Immunoprecipitation:Article Title: Optimal CXCR5 expression during Tfh maturation involves the Bhlhe40-Pou2af1 axis.
Article Snippet: Compound fisher healthcare Cat#23-730-571 VWR® Superfrost® Plus Micro Slide, Premium Avantor Cat#48311-703 PBS, pH 7.4 ThermoFisher Cat#10010031 Fluoromount G Southern Biotech Cat#0100-01 Coating buffer (pH = 9.5) BD Cat#51-2713KC Stop Solution invitrogen Cat#SS04 normal mouse serum Jackson immuno Research Cat#015-000-120 (Continued on next page) Cell Reports 44, 116470, November 25, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER normal rat serum Jackson immuno Research Cat012-000-120 IL-2 R&D systems Cat#401-ML IL-12 PeproTech Cat#210-12 IL-4 R&D Systems Cat#404-ML TGFβ1 R&D systems Cat#7666-MB IL-6 PeproTech Cat#216-16 IL-1β R&D systems Cat#401-ML Fixable Viability Dye eFluor TM 506 eBioscience Cat# 65-0866-18 Fixable Viability Dye eFluor TM 780 eBioscience Cat#65-0865-14 Dynabeads TM Mouse T-Activator CD3/ CD28 for T cell Expansion and Activation Thermofisher Cat#11452D UltraComp eBeads TM Compensation Bead Thermofisher Cat#01-2222-41 Phorbol 12-myristate 13-acetate Sigma Cat#P8139-5MG Ionomycin calcium salt from Streptomyces conglobatus Sigma Cat#I0634-5MG Monensin Solution (1000×) eBioscience Cat#00-4505-51 Critical commercial assays IgG1 Mouse Uncoated ELISA Kit with Plates invitrogen Cat#88-50410-86 IgG2c Mouse Uncoated ELISA Kit with Plates invitrogen Cat#88-50670-22 IgG (Total) Mouse Uncoated ELISA Kit with Plates invitrogen Cat#88-50400-86 miRNeasy micro kit Qiagen Cat#217084 DNase set Qiagen Cat#79254 QubitTM RNA High Sensitivity (HS), Assay Kit ThermoFisher Cat#Q10210 QubitTM dsDNA Broad Rang (BR) Assay Kit ThermoFisher Cat#Q32850 Dynabeads mRNA DIR ECT kit Ambion Life Technologies Cat#61012 EasySep TM Mouse Naive CD4 + T cell Isolation Kit STEMCELL Cat#19765 BD OptEIA TM Reagent Set Bt B BD Cat#550534 Foxp3 staining buffer set eBioscience Cat#00-5523-00 Deposited data RNA-Seq Raw and analyzed data This paper GSE247137 ChIP-Seq Raw and analyzed data This paper GSE247137 Experimental models: Organisms/strains Mouse: Pou2af1 − /− , This paper N/A Mouse: Pou2af1-HA-tdTomato This paper N/A Mouse: Bhlhe40 − /− This paper N/A Mouse: Bhlhe40-V5 This paper N/A Mouse: Bhlhe40 fl/fl –CD4Cre This paper Yu et al. 24 Mouse: Pou2af1 tm1a(KOMP)Wtsi the University of California, Davis, KOMP Repository www.mmrrc.org Mouse: CD45.1 + NIAID-Taconic repository Line 7 Mouse: Rag1 − /− NIAID-Taconic repository Line 146 Mouse: TCRα − /− NIAID-Taconic repository Line 98 Mouse: Cas9 JAXSON JAX 026179 Mouse: OT-II NIAID-Taconic repository Line 187 (Continued on next page) 18 Cell Reports 44, 116470, November 25, 2025 ..
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